The Italian centre-back is one of the key components of Max Allegri's Juve side and this injury will cause major concern for the Italian champions with their Champions League clash with Real Madrid just 17 days away.
Chiellini sustained the injury after landing awakwardly following an aerial challenge and based on the experienced defender's reaction, he appeared to have suffered a strain or tear of his right thigh.
Juve didn't take any chances and immeidately withdrew their star man, sending on Andrea Barzagli in his place.
The extent of the injury will remain shrouded in mystery until Chiellini can undergo a scan in the coming days.
The defender is one of Juve's most reliable players, having made 32 appearances this season, playing 90 minutes in all but two of those games.
With the repeat of last season Champions League final a little over two weeks away, Chiellini's race to be fit begins in earnest.